sara Ataei Nazari; abdollah mohammadi sang cheshmeh; Mohammad Reza Bakhtiarizadeh; ali assadi-alamouti; Ali Fouladi Nashta
Volume 22, Issue 3 , September 2020, , Pages 491-500
Abstract
This study was conducted to evaluate the effect of various concentrations of lipopolysaccharide (LPS) in maritaruta medium on oocyte maturation, oocyte developmental competence and metabolites related to maturation medium including glucose, pyruvate, lactate and glutamine. The experimental ...
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This study was conducted to evaluate the effect of various concentrations of lipopolysaccharide (LPS) in maritaruta medium on oocyte maturation, oocyte developmental competence and metabolites related to maturation medium including glucose, pyruvate, lactate and glutamine. The experimental treatments were 0 (control), 0.01, 0.1, 1 and 10 μg/ml of LPS in oocyte maturation medium. The cumulus oocytes complex (COC) were cultured with various concentrations of lipopolysaccharide. After 24 h of oocyte maturation, the medium was collected and the rates of oocyte maturation, cleaved oocyte and oocytes reached to blastocyst stage were analyzed. Oocyte maturation rate was lowest in the treatment with 10 μg/ml of LPS (P<0.05). Among the measured metabolites, only glucose concentration was linearlydecreased in response to increasing levels of LPS in the maturation medium (P<0.05) as treatment with 10 μg/ml of LPS had lower glucose concentration comparing to other treatments. The percentage of oocyte cleavage was significantly lower in treatment with highest level of LPS compared to other treatments (P<0.05). In addition, the treatment with 1 and 10 μg/ml of LPS significantly reduced blastocyst rate compared to control group (P<0.05). According to results of this study, lipopolysaccharide could have detrimental effects on oocyte development and these influences seems to be mediated through pathways related to energy metabolism. Acquiring managerial approaches to control LPS enhancing agents during reproductive season could prevent animal's reproductive failure.
mahdi dehghani-sanij; Mohammad Ali Norouzian; Ahmad Afzalzadeh; ali assadi-alamouti
Volume 22, Issue 2 , June 2020, , Pages 211-221
Abstract
To study the effects of feeding rumen-protected ascorbic acid and salicylic acid on performance of dairy cows under heat stress conditions, forty four lactating dairy cows in early lactation were allocated in a completely randomized design with 4 treatments and 11 animals in each treatment for 30 days. ...
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To study the effects of feeding rumen-protected ascorbic acid and salicylic acid on performance of dairy cows under heat stress conditions, forty four lactating dairy cows in early lactation were allocated in a completely randomized design with 4 treatments and 11 animals in each treatment for 30 days. Experimental treatments were: 1) control (diet without coated ascorbic acid and salicylic acid), 2) diet contained 30 g/d coated ascorbic acid, 3) diet contained 10 g/d coated salicylic acid and 4) diet contained 30 g/d coated ascorbic acid + 10 g/d coated salicylic acid. Cows were fed with salicylic acid supplement had a higher daily milk yield (51.47 kg/day) compared to other experimental groups (P<0.05). Feeding of coated ascorbic acid increased milk fat percent, glucose, BHBA, NEFA concentration and decreased somatic cell count compared to other treatments (P<0.05). Total milk's solids, energy and fat corrected milk and milk urea nitrogen were increased in coated ascorbic acid group compared to control (P<0.05). Blood urea concentration in coated ascorbic acid and salicylic acid group were less than control group (P<0.05). The results showed that feeding coated ascorbic acid and salicylic acid could improve performance of Holstein dairy cows in early lactating phase under heat stress conditions.
Vahab Azimzadeh; Mehdi Dehghan banadaky; armin tohidi; ali assadi-alamouti
Volume 22, Issue 1 , March 2020, , Pages 47-54
Abstract
Effect of different processing methods of corn grain were investigated in 36 Holstein suckling female calves (body weight = 38.2 ± 1.20 kg) on performance and blood and ruminal parameters. Treatments were whole corn, ground corn, cracked corn and steam-flaked corn. Starter diets were ...
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Effect of different processing methods of corn grain were investigated in 36 Holstein suckling female calves (body weight = 38.2 ± 1.20 kg) on performance and blood and ruminal parameters. Treatments were whole corn, ground corn, cracked corn and steam-flaked corn. Starter diets were mixed five percent of the chopped wheat straw and offered to calves from day 4. Milk was fed in a gradual step-up step-down method to all calves. Calves weaned at 71 days and total intake of starter and body weight of calves were measured until 84 days. Starter intake and ADG were significantly higher for cracked corn and steam-flaked corn than for ground corn and whole corn treatments in pre- and post-weaning and overall period (P≤0.01). At weaning and day 84, the average body weight of calves fed cracked corn and steam-flaked corn were significantly higher than those fed whole corn and ground corn treatments (P≤0.01). Treatments also affected serum protein of calves (P<0.05). According results of this study, feeding calves with cracked corn or steam-flaked corn increased feed intake and improved ADG and body weight through pre- and post-weaning periods.
Masoud Norozi-ِDeeyarjan; ali assadi-alamouti; Ahmad Afzalzadeh; mohsen danesh mesgaran
Volume 19, Issue 4 , March 2018, , Pages 751-764
Abstract
This study examined the effects of partial replacement of soybean meal (SBM) with heat-treated soybean meal (HSBM) in heat-stressed cows. 32 multiparous Holstein cows (DIM = 110 ± 20; milk yield = 40 ± 4.1 kg) were used in a complete block randomized design with a 2×2 factorial arrangment ...
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This study examined the effects of partial replacement of soybean meal (SBM) with heat-treated soybean meal (HSBM) in heat-stressed cows. 32 multiparous Holstein cows (DIM = 110 ± 20; milk yield = 40 ± 4.1 kg) were used in a complete block randomized design with a 2×2 factorial arrangment of treatments. Treatments included two dietary crude protein levels (i.e. 17 and 15.5%) each combined with HSBM partially replaced for SBM. The experiment was conducted in two periods of 28 days with the last seven days for sampling and collection of data of tempreature, nutrient intake and digestibility and blood variables. Average THI showed that cows were in mild heat stress (70.60 and 71.50 for periods 1 and 2, respectively)(p > 0.05). Diets with 15.5% CP and based on HSBM increased intakes of dry and organic matter (p < 0.05); however, this was not observed in diets with 17% CP. Milk yiled and composition, apparent nutrient digestibility and blood variables were unaffected (p > 0.05). Butyric and isovaleric acid were increased in cows received HSBM (p < 0.05); however, level of CP had no effect. Results showed that feeding HSBM in diets with 15.5% CP could be regarded as a nutritional sterategy for improving DM intake of mild heat-stressed cows.
Somayeh Fathi; ali assadi-alamouti; Ahmad Afzalzadeh; Mohammad Ali Norouzian
Volume 19, Issue 3 , November 2017, , Pages 545-555
Abstract
The aim was to evaluate effects of in vitro fermentation of different forages co-incubated with different types of non-fiber carbohydrates (NFC) on gas production, digestibility, microbial biomass, medium pH and ammonia concentration. A completely randomized design with factorial arrangement (9 ...
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The aim was to evaluate effects of in vitro fermentation of different forages co-incubated with different types of non-fiber carbohydrates (NFC) on gas production, digestibility, microbial biomass, medium pH and ammonia concentration. A completely randomized design with factorial arrangement (9 treatments and 3 replicates) were used wherein wheat straw, alfalfa hay and corn silage constituted main forage sources and starch, sucrose and pectin were components of NFC. 0.2 g of each forage samples incubated with 0.3 g of each NFC component for 24 h and gas production, apparent and true digestibility, microbial biomass, pH and ammonia concentration measured. Forage and NFC sources, alone but not in combination, had a significant effect on gas production, digestibility as well as ammonia concentration (p<0.05). The estimated microbial biomass was lower for wheat straw samples (0.14 vs. 0.16 g /g DM digested for other forage samples), but was not affected by NFC and its interaction with forage sources (p<0.05). Also, NFC sources affected medium pH significantly with the lowest values for sucrose while the highest for pectin. Results showed that previously known effects of NFC sources in vivo are also consistently observed in vitro while it was not affected by co-incubation with different forage sources.
Somayeh Moeinizadeh; Ali Akbar Khadem; Ali Assadi-Alamouti; Ahmad Afzalzadeh
Volume 15, Issue 1 , July 2014, , Pages 31-43
Abstract
The objectives were to investigate the effects of addition of alfalfa hay as an absorbent on fermentationquality and effluent production of corn silage. Three levels of corn forage with dry matter (DM) contentsof 18.3, 21.7 and 25.5 percent were supplemented with 0, 5, 10, and 20 percent alfalfa hay, ...
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The objectives were to investigate the effects of addition of alfalfa hay as an absorbent on fermentationquality and effluent production of corn silage. Three levels of corn forage with dry matter (DM) contentsof 18.3, 21.7 and 25.5 percent were supplemented with 0, 5, 10, and 20 percent alfalfa hay, on as fedbasis, and ensiled in three replicates in polyvinyl chloride mini-silos in a completely randomized design.Concentrations of ash and total nitrogen increased and that of neutral detergent fiber decreasedsignificantly as the level of absorbent increased (P< 0.05). Increasing the level of absorbent significantlyincreased the DM of silage where the higher levels of DM were observed in silages with 10 and 20percent absorbent. The pH values were within the optimum range of corn silage (ranging from 3.79 to4.33); however, silage pH was affected by the interaction between DM and level of absorbent (P<0.05).The pH increased as the level of absorbent increased, however, the effect was less observed in silageswith 26 percent DM. Also, there was an interaction between the DM and absorbent in the concentration ofresidual water-soluble carbohydrate of silage where the differences in water-soluble carbohydrates insilages with 18 percent initial DM were lower than those in silages with 22 and 26 percent DM. As thelevel of absorbent increased, aerobic stability was improved with the effects becoming less pronounced asthe level of DM increased. Effluent production was markedly reduced as the level of absorbent increasedso that it dropped to 0 ml at 20 percent alfalfa hay addition at all DM levels (P<0.05). The concentrationsof DM and nitrogen were higher in effluent produced from silages treated with higher level of absorbent;however, total DM and nitrogen losses were lower due to considerably lower effluent production. Resultsshowed that using a minimum of 10 percent alfalfa hay could effectively reduce effluent production andimprove aerobic stability of corn silage while maintaining its optimal pH.